Head of the biotechnology educational laboratory, Department of Biochemistry and Biotechnology, Vasyl Stefanyk Carpathian National University (CNU)
Research profiles
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I. PERSONAL/CONTACT DETAILS
Ukrainian, born July 18, 1993
Work address: Department of Biochemistry and Biotechnology, Vasyl Stefanyk Carpathian National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
Phone: +38(0342)596171
E-mail: mariia.lylyk@cnu.edu.ua, marialylyk@ukr.net
Languages: Ukrainian, English
Hobbies: yoga, reading, travelling, reuse/recycling
II. EDUCATION
2015-2018 PhD student, Department of Biochemistry and Biotechnology, PNU. PhD thesis in Biochemistry: “Protective effect of alpha-ketoglutarate in fruit flies Drosophila melanogaster under the influence of xenobiotics”, defended at Ivan Gorbachevsky Ternopil National Medical University, Ukraine (November, 2022). Supervisor: Prof. Maria Bayliak, Head of Department of Biochemistry and Biotechnology, PNU.
2014–2015 Master’s Diploma in Biochemistry. M.Sc. Thesis (in Biochemistry): Resistance to oxidative stress and indices of antioxidant protection in Drosophila melanogaster Canton S grown on medium with alpha-ketoglutarate. Supervisor: Dr. Maria Bayliak of Department of Biochemistry and Biotechnology, PNU.
2010–2014 – Bachelor Diploma in Biology (Department of Biochemistry and Biotechnology, Faculty of Natural Sciences, Vasyl Stefanyk Precarpathian National University, Ivano- Frankivsk, Ukraine).
III. TEACHING EXPERIENCE
September 2019-June 2021, 2024-2025 – Assistant (part time), Biochemistry and Biotechnology Dept., CNU. Practical courses: “Large workshop on laboratory diagnostics”, “Biologically active natural compounds”, “Basis of Biochemistry”, “Microbiology”, “Virology”, “Molecular Biology” “Physiology and biochemistry of plants”, “Biochemistry with the basics of microbiology and virology”, ” Basis of clinical biochemistry”, “Bioethics and Biosafety”, “Microbial Biotechnology”.
IV. PROFESSIONAL EXPERIENCE / TRAINING / SCHOLARSHIP:
2024-2025 – Teacher of the courses “Bioethics and biosafety: practical aspects” funded by the German Academic Exchange Service (DAAD)
01/2025 – present Senior researcher in the project funded by Ministry of Education and Science of Ukraine: “Preclinical evaluation of the efficacy of pharmaceuticals for alleviating the effects of post-traumatic stress disorder (#0125U000396)”. Project leader – Ph..D O. Strilbytska
01/2023 – present Senior researcher in the project funded by Ministry of Education and Science of Ukraine: “Searching for markers of post-traumatic stress disorder based on oxidative stress and inflammation (#0123U101790)”. Project leader – Prof. M. Bayliak
01/2022 – 01/2024 Researcher in the project funded by Ministry of Education and Science of Ukraine: “Correction of metabolic syndrome with sulforaphane-enriched preparations from broccoli sprouts”. Project leader – Prof. V. Lushchak.
February 2021 – present Head of the biotechnology teaching laboratory, Department of Biochemistry and Biotechnology.
12/2020 – 12/2023 Researcher in the project of National Research Foundation of Ukraine “Phenylpropanoid pathway intermediates as substances to prolong the duration and quality of life” (#2020.02/0118). Project leader – Prof. M. Bayliak.
12/2019-12/2020 Researcher in the project funded by Ministry of Education and Science of Ukraine: “Development of new non-medicinal methods for correction of metabolic syndrome: normalization of physiological and biochemical indices in animals” (#0118U003477). Project leader – Prof. V. Lushchak.
June 6-30 2019 Attendance at DAAD Summer School “Perspectives in Biomedicine with a Focus on Cancer Immunotherapy”, CNU, Ivano-Frankivsk, Ukraine.
11/2018 – 2/2021 Leading specialist of the research laboratory, Department of Biochemistry and Biotechnology, CNU.
12/2017-01/2018 Researcher in the project “Study of novel milk formulas and devices on a premature pig model ensuring proper PUFA absorption and brain development in preterm infants” at the Kielanowski Institute of Animal Physiology and Nutrition (Jablonna, Poland). Project leader – Prof. Stefan Pierzynowski
12/2016-01/2017 Researcher in the scientific project at the Kielanowski Institute of Animal Physiology and Nutrition (Jablonna, Poland). Project leader – Prof. Stefan Pierzynowski.
July 4-8, 2016 Training at PolLASA courses on breeding, maintenance, welfare and use of laboratory animals (Polish Laboratory Animals Science Association, Warsaw, Poland)
V. LABORATORY METHODS (PROFICIENCY):
Microbiological methods: preparation of microscope slides of bacteria, light microscopy, cultivation of microorganisms, isolation of microbial cultures.
Physiological methods (on fruit fly D. melanogaster): maintaining of fly culture; lifespan assay; assays of age-related functional declines (climbing activity, resistance to stresses, fecundity, etc.).
Biochemical methods: preparation of tissue extracts from flies and mice, measurement of contents of main metabolites (glucose, glycogen, triacylglycerides, protein); enzyme activities assays (superoxide dismutase, catalase, glutamate dehydrogenase, glucose-6- phosphate dehydrogenase, alcohol dehydrogenase, aspartate aminotransferase, alanine aminotransferase, hexokinase, aconitase); protein electrophoresis; western blot; urine analysis; water analysis; ELISA assay; spectrophotometric determination of major markers of oxidative stress.
Lab management
VI. SCIENTIFIC INTERESTS AND CURRENT RESEARCH WORK
– Oxidative stress and antioxidant defense in living organisms
– Mechanisms of adaptation of living organisms to different stresses, in particular to oxidative stress
– Free radicals, antioxidants, adaptogens, geroprotectors, detoxifying natural compounds (alpha- ketoglutarate, arginine, etc.) and their influence on fruit fly Drosophila melanogaster
– Obesity as a result of high fat diet and obesity prevention strategies (on fruit fly and mouse models)
– Aging and anti-aging treatments
– Post-traumatic stress disorder in the mouse model
VII. AWARDS
Winner of the IV International conference «Drosophila in experimental genetics and biology» (best oral presentation). Lviv, 2015 year.
Diploma of the III degree of the Ukrainian competition of student’s scientific works on biological sciences. Chernivtsi, 2015 year.
VIII. OTHER ACTIVITIES
Participation in the competition of start-up projects Eco-Friendly from the regional accelerator GCIP for innovations, technologies and startups in Ivano-Frankivsk region, which was established on the basis of Vasyl Stefanyk Precarpathian National University with the support of the United Nations Industrial Development Organization (UNIDO).
Participation in the project “EcoREactive 2.0” for the collection of sorted plastic in the communities of the Carpathian region, organized by the “Mountain Rescue Center” in conjunction with the “Dniester Regional Landscape Park. Serhiy Didych” within the framework of the Direct Aid Program of the Government of Australia.
Membership in Ukrainian Biochemical Society
Member of Organizing Committee at Carpathian Summer School in Biochemistry held annually at Department of Biochemistry and Biotechnology, СNU
Member of Organizing Committee at Autumn School for Young Biochemists held annually at Department of Biochemistry and Biotechnology, СNU
IX. Publications
Papers: articles – 21. Abstracts – 19. h-index in the SCOPUS database – 9. Articles in journals Q1 and Q2 – 11 for the last 10 years (11 in total).
LIST OF PUBLICATIONS
Experimental articles in Q1 and Q2 journals
1. Vatashchuk M.V., Hurza V.V., Abrat O.B., Lylyk M.P., Matvieieva K., Gospodaryov D.V., Demianchuk O.I., Storey K.B., Bayliak M.M., Lushchak V. I. Alpha-ketoglutarate supplementation differently modifies hematological parameters and oxidative stress in mice fed a standard diet and high-fat high-fructose diet. J Physiol Biochem. 2025. https://doi.org/10.1007/s13105-025-01115-5 Q1 Biochemistry
2. Hurza V. V., Bayliak M. M., Vatashchuk M. V., Sorochynska O. M., Lylyk M. P., Abrat O. B., Gospodaryov D. V., Storey K. B., Lushchak V. I. Intermittent fasting partially alleviates dietary margarine-induced morphometrical, hematological, and biochemical changes in female mice, but not in males. Biochemistry Research International, Vol. 2025, Article ID 2163104, https://doi.org/10.1155/bri/2163104 Q2 Biochemistry
3. Demianchuk O., Lylyk M., Balatskiy V., Gospodaryov D., Bayliak, M. Alpha-ketoglutarate supplementation in long-lived Drosophila melanogaster: Impact on lifespan and metabolic responses. Archives of Insect Biochemistry and Physiology. 2024, 116(1), e22116 https://doi.org/10.1002/arch.22116. Q2 Insect Science
4. Bayliak M.M., Demianchuk O. I., Gospodaryov D.V., Abrat O.B., Lylyk M.P., Storey K.B., Lushchak V.I. Mutations in genes cnc or dKeap1 modulate stress resistance and metabolic processes in Drosophila melanogaster // Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2020. Vol. 248. https://doi.org/10.1016/j.cbpa.2020.110746 Q1 Animal science and Zoology
5. Bayliak M.M., Lylyk M.P., Gospodaryov D.V., Kotsyubynsky V.O., Butenko N.V., Storey K.B., Lushchak V.I. Protective effects of alpha-ketoglutarate against aluminum toxicity in Drosophila melanogaster. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2019. 217, 41-53. https://doi.org/10.1016/j.cbpc.2018.11.020 Q2 Biochemistry
6. Bayliak M.M., Lylyk M.P., Maniukh O.V., Storey J.M., Storey K.B., Lushchak V.I. Dietary L-arginine accelerates pupation and promotes high protein levels but induces oxidative stress and reduces fecundity and lifespan in Drosophila melanogaster. J. Comp. Physiol. B. 2018. 188(1), 37-55. https://doi.org/10.1007/s00360-017-1113-6 Q1 (Animal Science and Zoology)/Q2 Biochemistry
7. Bayliak M.M., Lylyk M.P., Shmihel H.V., Sorochynska O.M., Semchyshyn O.I., Storey J.M., Storey K.B., Lushchak V.I. Dietary alpha-ketoglutarate promotes higher protein and lower triacylglyceride levels and induces oxidative stress in larvae and young adults but not in middle-aged Drosophila melanogaster. Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol. 2017. 204, 23-33. https://doi.org/10.1016/j.cbpa.2016.11.005 Q2 Biochemistry
8. Bayliak M.M., Shmihel H.V., Lylyk M.P., Storey K.B., Lushchak V.I., 2016. Alpha- ketoglutarate reduces ethanol toxicity in Drosophila melanogaster by enhancing alcohol dehydrogenase activity and antioxidant capacity. Alcohol. 55, 23-33. https://doi.org/10.1016/j.alcohol.2016.07.009 Q1 Toxicology/ Q2 Biochemistry
9. Bayliak M.M., Lylyk M.P., Vytvytska O.M., Lushchak V.I., 2016. Assessment of antioxidant properties of alpha-keto acids in vitro and in vivo. Eur. Food Res. Technol. 242 (2), 179-188. https://doi.org/10.1007/s00217-015-2529-4 Q1 Food science/ Q2 Biochemistry
10. Bayliak M.M., Lylyk M.P., Shmihel H.V., Sorochynska O.M., Manyukh O.V., Pierzynowski S. G., Lushchak V.I., 2016. Dietary alpha-ketoglutarate increases cold tolerance in Drosophila melanogaster and enhances protein pool and antioxidant defense in sex-specific manner. J. Therm. Biol. 60, 1-11. https://doi.org/10.1016/j.jtherbio.2016.06.001 Q1 Agricultural and Biological Sciences (miscellaneous)
11. Bayliak M.M., Shmihel H.V., Lylyk M.P., Vytvytska O.M., Storey J.M., Storey K.B., Lushchak V.I., 2015. Alpha-ketoglutarate attenuates toxic effects of sodium nitroprusside and hydrogen peroxide in Drosophila melanogaster. Environ. Toxicol. Pharmacol. 40(2), 650-659. https://doi.org/10.1016/j.etap.2015.08.016 Q2 Toxicology
Other articles
1. Balatskyi V., Strilbytska O., Abrat O., Tkachyk A., Lylyk M., Lushchak V., Bayliak M. Behavioral and metabolic effects of escapable electric foot shock stress in male mice. BMC Research Notes. 2025, 18:127, doi:10.1186/s13104-025-07189-0 Q3 Biochemistry, Genetics and Molecular Biology (miscellaneous)
2. Strilbytska, O., Hurza, V., Vatashchuk, M., Derkachov, V., Berezovskyi, V., Demianchuk, O., Lushchak, O., Lylyk, M., Bayliak, M. (2025). Behavioral and Molecular Effects of Magnesium and Vitamin B6 Complex in a Mouse Model of Post-Traumatic Stress Disorder. Journal of Vasyl Stefanyk Precarpathian National University. Biology, 12, 60–73. https://doi.org/10.15330/jpnubio.12.60-73
3. Hurza, V., Butenko N., Demianchuk O., Balatskiy V., Derkachov V., Lylyk M. 2023. High Consumption of Lard and Fructose Modulates Larval Pupation and Stress Resistance in Drosophila Melanogaster Adults. Journal of Vasyl Stefanyk Precarpathian National University. 9, 4 (Jan. 2023), 42–55. doi:https://doi.org/10.15330/jpnu.9.4.42-55.
4. Lylyk M.P., Bayliak M.M., Shmihel H.V., StoreyJ.M., Storey K.B., Lushchak V.I. Effects of alpha-ketoglutarate on lifespan and functional aging of Drosophila melanogaster flies. Ukr.Biochem.J. 2018. Vol. 90 (6), 49-61. https://doi.org/10.15407/ubj90.06.049
5. Bayliak M.M., Lylyk M.P., Sorochynska O.M. Dietary alpha-ketoglutarate partially prevents age-related decline in locomotor activity and cold tolerance in Drosophila melanogaster. Biologia. 2017. Vol. 72. N4. P. 458-467. https://doi.org/10.1515/biolog-2017-0042
6. Lylyk M. P., Golovchak M. V., Shmihel H. V., Bayliak M. M. 2017. Influence of alpha- ketoglutarate on Drosophila melanogaster resistance to different toxicants. Journal of Medicine, Biology and Sports. Vol. 4. N 6. P. 180-185. 10.26693/jmbs02.04.180
7. Lylyk M., Sorochynska O., Maniukh O., Bayliak M. 2017. Age-related physiological and biochemical changes in Drosophila grown on alpha-ketoglutarate. Bulletin of Taras Shevchenko National University of Physiological Functions Regulation. 22(1), 25-31.
8. Lylyk M., Sorochynska O., Maniukh O., Bayliak M. 2016. Gender differences of amino acid metabolism in Drosophila melanogaster on alpha-ketoglutarate-supplemented food. Bulletin of Taras Shevchenko National University of Physiological Functions Regulation. 21(2), 31-36.
9. Lylyk M.P., Bayliak M.M. 2015. Possible protective mechanisms of alpha-ketoglutarate on fruit fly Drosophila melanogaster Canton S under exposure to different stressors. Biological systems. 7(1). 119-123.
10. Shmihel H., Lylyk M., Bayliak M. 2014. Effect of alpha-ketoglutarate on pupation, feeding intensity and levels of some metabolites in larvae of Drosophila melanogaster. Visnyk of the Lviv University. Series Biology. 2014. 66, 91–99.
Abstracts
1. Gospodarov D., Demyanchuk O., Lylyk M., Bayliak M. The effect of a high-calorie diet with fructose and α-ketoglutarate on the regulation of energy metabolism in the liver and brain of mice. Chargaff Readings: Proceedings of the XX International Scientific Conference dedicated to the 150th anniversary of Yuri Fedkovych Chernivtsi National University and the 120th anniversary of Erwin Chargaff’s birth (Chernivtsi, October 27–30, 2025). Chernivtsi: Yuri Fedkovych Chernivtsi National University, 2025. P. 175–176.
2. Gospodarov D., Demyanchuk O., Lylyk M., Bayliak M. Influence of mutations in the cnc and dKeap1 genes on antioxidant protection and metabolism in Drosophila melanogaster fruit flies. Drosophila in experimental genetics and biology: collection of abstracts from the IX International Conference (Svitiaz, June 16–18, 2025). Lutsk, 2025. P. 12.
3. Lylyk M., Dmytriv T., Melnychuk A., Bayliak M. Influence of chamomile aqueous extract on the stress resistance, antioxidant defence and fecundity in middle-aged Drosophila melanogaster // Drosophila in experimental genetics and biology: Abstract book of the 8th International conference (Ivano-Frankivsk, June 27–29, 2023). – P.33.
4. Demianchuk O., Lylyk M., Gospodaryov D. The effect of exogenous alpha-ketoglutarate on metabolic indicators in the body of Drosophila melanogaster // Drosophila in experimental genetics and biology: Abstract book of the 8th International conference (Ivano-Frankivsk, June 27–29, 2023). (Ivano-Frankivsk, 27-29 червня, 2023). – P.27.
5. Bayliak M., Demianchuk O., Lylyk M., Gospodaryov D. The effect of alpha-ketoglutarate and Rhodiola rosea preparations on energy metabolism indicators in middle-aged Drosophila melanogaster // Drosophila in experimental genetics and biology: Abstract book of the 8th International conference (Ivano-Frankivsk, June 27–29, 2023). – P.28.
6. Demianchuk O., Lylyk M., Shmihel H., Gospodaryov D., Bayliak M. The effect of exogenous alpha-ketoglutarate on the lifespan and antioxidant protection of Drosophila melanogaster // Abstracts of the III International Scientific and Practical Conference “Problems and Achievements of Modern Biotechnology”, (Kharkiv, March, 24, 2023). – P.161-162.
7. Demianchuk O., Gospodaryov D., Vatashchuk M., Lylyk M., Bayliak M. Effect of a diet high in fat and fructose on glycolysis and fructolysis and the corrective effect of alpha- ketoglutarate // Abstracts of the XX All-Ukrainian Scientific and Practical Conference of Young Scientists (May 19, 2022, Lviv).
8. Dmytriv Т., Strilets N., Lylyk M. The content of phenolic compounds and antioxidant properties of water and ethanol exracts from Matricaria chamomilla flowers // ХVI International Scientific Conference “Youth and Progress of Biology”, Lviv, 2020. P. 34-35.
9. Derkachov V., Berezovskyi V., Dmytriv Т., Strilets N., Lylyk M. Effects of water exracts from Matricaria chamomilla flowers on food intake and climbing actiivty in Drosophila melanogaster // ХVI International Scientific Conference “Youth and Progress of Biology”, Lviv, 2020. P. 248-249.
10. Strilets N., Dmytriv T., Lylyk M., Bayliak M. Influence of animal and plant fats on storage lipid acumulation in Drosophila melanogaster // ХVI International Scientific Conference “Youth and Progress of Biology”, Lviv, 2020. P. 253-254.
11. Butenko N.V., Lylyk M.P., Gospodaryov D.V., Bayliak M.M. Alpha-ketoglutarate alleviates aluminum toxicity in Drosophila melanogaster // Medical and clinical chemistry “Materials of the XII Ukrainian Biochemical Congress, Ternopil, September 30-October 4, 2019”. V. 21, N3 (supplement). P. 65.
12. Gospodaryov D., Lylyk M., Demianchuk O., Sitko M., Yurchak T., Bayliak M. Deficiency in Nrf2 and Keap1 delays development and modulates metabolic processes in Drosophila melanogaster // 26th European Drosophila Research Conference (5th-8th September 2019, EPFL, Switzerland). Program & Abstract Book. P. 196-197.
13. Ivasyshyn V., Butenko N., Lylyk M. Combined influence of alpha-ketoglutarate and aluminum ions on resistance of Drosophila melanogaster to different stressors // XIII International Scientific Conference “Youth and Progress of Biology”, Lviv, April 25-27, 2017. P. 252-253.
14. Semchyshyn O., Lylyk M., Bayliak M. Effect of alpha-ketoglutarate on cold stress resistance of Drosophila melanogaster at different ages // XII International Scientific Conference “Youth and Progress of Biology”, Lviv, April 19-21, 2016.
15. Lylyk M.P., Bayliak M.M. Influence of alpha-ketoglutarate on the general physiological activity of 24-day-old Drosophila melanogaster // V International Scientific Conference “Drosophila in Experimental Genetics and Biology”, Kyiv, May 12-14, 2016.
16. Lylyk M.P. Mechanisms of protective action of alpha-ketoglutarate on two-day-old individuals of Drosophila melanogaster under the influence of cold stress // Ukr. Biochem. J., 2016, Vol. 88, N 4, P 85, ISSN 2409-4943.
17. Lylyk M., Vytvytska О. The effect of alpha-ketoglutarate on the resistance of Drosophila melanogaster to cold stress // XI International Scientific Conference “Youth and Progress of Biology”, Lviv, April 20-23, 2015.
18. Lylyk M., Shmihel H., Kozachok O., Bayliak M. Alpha-ketoglutarate modifies toxic action of sodium nitroprusside and ethanol on Drosophila melanogaster // Ukr. Biochem. J. Vol. 86, N 5, supplement 2 “Materials of XI Ukrainian Biochemical congress, Kyiv, October 6- 10, 2014”. P 249-250.
19. Lylyk M., Vytvytska О. Influence of alpha-ketoglutarate on indices of physiological activity of Drosophila melanogaster // VIII International conference of young scientists “Biology: from molecule to biosphere”, Kharkiv, 2013. P 85-86.